TB-500 (Thymosin Beta-4 Fragment)
A synthetic peptide representing the active region of Thymosin Beta-4, studied for its role in actin regulation, cell migration, and tissue repair mechanisms.
Overview
TB-500 is a synthetic version of the active region of Thymosin Beta-4 (Tβ4), a 43-amino-acid protein that is naturally present in nearly all human and animal cells. Thymosin Beta-4 was first isolated from calf thymus tissue and is one of the most abundant members of the beta-thymosin family.
The key functional domain of Thymosin Beta-4 is the central actin-binding sequence (amino acids 17-23: LKKTETQ), which is the region replicated in TB-500. This sequence has been the focus of extensive research into cytoskeletal dynamics, cell motility, and wound repair.
Molecular Profile
Mechanism of Action
TB-500's biological activity centres on its interaction with monomeric actin (G-actin), which drives several downstream effects:
- Actin sequestration: TB-500 binds G-actin in a 1:1 complex, regulating the polymerisation of actin filaments. This is central to cell motility and structural remodelling (Safer et al., 1997).
- Cell migration promotion: Research has demonstrated that Thymosin Beta-4 promotes the migration of endothelial cells, keratinocytes, and other cell types, which is relevant to angiogenesis and wound closure research (Malinda et al., 1999).
- Anti-inflammatory pathways:Preclinical studies have observed a reduction in pro-inflammatory cytokines in models treated with Tβ4, suggesting involvement in inflammatory modulation (Sosne et al., 2010).
- Extracellular matrix interaction:Tβ4 has been shown to influence matrix metalloproteinase activity, which plays a role in tissue remodelling and repair processes (Goldstein et al., 2005).
Key Published Research
Thymosin beta4 activates integrin-linked kinase and promotes cardiac cell migration, survival and cardiac repair
Bock-Marquette I, et al. Nature. 2004; 432:466-472
Landmark study published in Nature demonstrating Tβ4's activation of integrin-linked kinase (ILK) and its effects on cell survival pathways in cardiac research models.
Thymosin beta4 promotes dermal healing
Philp D, et al. Annals of the New York Academy of Sciences. 2007; 1112:413-419
Study examining Tβ4's effects on full-thickness dermal wound models, including cell migration, collagen deposition, and angiogenesis.
Storage and Handling
Lyophilised (unreconstituted):Store at -20°C for long-term stability. Stable at 2-8°C for shorter periods. Protect from light.
Reconstituted:Store at 2-8°C. Use within 30 days. Avoid repeated freeze-thaw cycles.
Recommended solvent: Bacteriostatic water or sterile water for laboratory use.
Frequently Asked Questions
What is the difference between TB-500 and Thymosin Beta-4?
Thymosin Beta-4 is the full 43-amino-acid protein. TB-500 is a synthetic peptide that contains the active region responsible for actin binding and cell migration. In research contexts, the terms are often used interchangeably, though they are structurally distinct.
What research areas use TB-500?
TB-500 has been studied in wound healing research, cardiovascular biology, ophthalmology (corneal repair models), and musculoskeletal research. Its primary mechanism of interest is actin regulation and cell migration.
Disclaimer: This information is compiled from published peer-reviewed literature and is provided for educational and research reference purposes only. It does not constitute medical advice. TB-500 sold by Enhanced Research Compounds is intended exclusively for in-vitro research and laboratory use. It is not a therapeutic good, is not listed on the ARTG, and is not approved for human or animal consumption.